Exploring the treatment of epilepsy through intrahippocampal GABA modulation with an HSV-vector expressing GAD67
نویسندگان
چکیده
This study explores the effect of a herpes-simplex viral (HSV) vector expressing glutamic acid decarboxylase 67 (GAD67) in the rat hippocampus. GAD67 gene transfer increases GABA release in transfected neurons by 3 days and its effect diminishes over 3 weeks. Transgene expression was associated with a decrease in voltage-gated sodium channel α-subunits 1.2 (NaV1.2) and 1.6 (NaV1.6) in the hippocampus. Changes in VGSC levels, along with enhanced GABA neurotransmission, are likely responsible for the prevention of status epilepticus in rats expressing the GAD67 transgene using a pilocarpine-based model of epilepsy. Thus, gene transfer of GAD67 under a latency promoter for prolonged expression may have therapeutic value for patients with intractable epilepsy.
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